Glucose Tolerance Tests describes a laboratory practical which enables students to investigate tolerance to a glucose load in obese (leptin deficient) and wild-type mice. It comprises of five elements: taking and analysis of basal and post-glucose blood samples; IP injection of glucose; post-mortem dissection of fat pads; post experiment analysis of blood & tissue samples. All procedures and equipment required for running this practical are clearly described.
This protocol was developed by Dr King and colleagues from Kings College London, UK. It is published in the Journal of Pharmacological and Toxicological Methods (2011) 64 (2): 168-172, with copyright being retained by the publishers.
It is suitable for use in undergraduate student education and short training courses in in-vivo sciences.
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