ETRIS: Education & Training Resources in In-Vivo Sciences

Experimental and statistical design

Assay Capability Tool

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106108

Assay Capability Tool is an experimental design tool to facilitate the design and development of new, robust and reproducible experimental protocols and assays in in-vivo research.

There is a high risk of experimental bias and irreproducibility in the majority of preclinical research areas. The Assay Capability Tool consists of a set of key experimental design questions including randomisation, sample size calculations, blinded outcome assessments and statistical analyses which users must consider in designing new assays or protocols. Its use in the design of new assays and protocols will increase the validity, quality and reproducibility of the data produced and reduce animal usage.

Details of this tool and its use in the development of in-vivo assays for evaluating the efficacy of new compounds in pre-clinical (pain) research are published in:

Miranda JA et al. (2014). A Preclinical Physiological Assay to Test Modulation of Knee Joint Pain in the Spinal Cord: Effects of Oxycodone and Naproxen. PLoS ONE 9 (8): e106108. doi:10.1371/journal.pone.0106108.

Copyright of this article is retained by the Journal.

This resource will be of use to researchers involved in the design of research studies or novel assays.

Other resources in this category:

  • 3Rs-Reduction - view
  • ARRIVE guidelines - view
  • Assay Capability Tool - view
  • EMPReSS - view
  • Experimental Design Assistant - view
  • InVivoStat - view
  • Reproducibility and reliability of biomedical research - view
  • Reproducibility in Research - view
  • Systematic Reviews of Animal Studies - view

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