ETRIS: Education & Training Resources in In-Vivo Sciences

Educational resources and protocols

Renal response to volume expansion

Renal response to volume expansion describes a laboratory practical which enables students to investigate the renal response to volume expansion in anaesthetised rats. It comprises of three elements: an interactive preparatory tutorial on the regulation of body fluids; a laboratory session in which students collect blood and urine samples during volume expansion in anaesthetised rats (surgically pre-prepared by instructors), with post experiment analysis of blood and urine volumes and composition; data analysis and interpretation session. All procedures and equipment required for running this practical are clearly described.

This protocol was developed by Dr Kline and colleagues from the University of Western Ontario, Canada. It is published in the journal Advances in Physiology Education (2000) 23 (1): 24-31, with copyright being retained by the American Physiological Society.

It is suitable for use in undergraduate student education and short training courses in in-vivo sciences.

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