In-vivo time-lapse imaging in the Zebrafish lateral line: A flexible, open-ended research project for an undergraduate Neurobiology laboratory course is a journal article describing a laboratory session in which students investigate aspects of axonal development in zebrafish.
This article describes the preparation of the animals, experimental equipment and resources required and procedures, student protocols and data analysis. In this laboratory, students learn to handle and image living fish, collect time-lapse videos of moving mitochondria, and quantitatively measure mitochondrial dynamics by generating and analysing kymographs of their movements. Use of these procedures in undergraduate research projects is also described.
It was developed by Dr Marra and colleagues, Lewis & Clark and Reed Colleges, Oregan, USA and is published in the Journal of Undergraduate Neuroscience Education (Marra et al. (2015) JUNE 13 (3): A215-A224). Copyright is retained by the journal.
This laboratory session can be used in undergraduate student education as part of a larger in-vivo neurobiology course.
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